Fig. 3. Mitochondria-targeted Kv1.3 inhibitors induce a dose- and time-dependent cell death of Jurkat leukemia cells. Jurkat cells were left untreated or treated with 0.25 mM, 1 mM or 10 mM PCARBTP for 24, 48, 72 or 96 hrs. Cell death was determined by Trypan Blue staining (a) or FITC-Annexin V staining and flow cytometry analysis (b). Panel c shows an example of the flow cytometry analysis. The fluorescence intensity is given in arbitrary units (a.u.). Given are the mean ± SD of cell death in percent from total cells per sample; n=4, *p<0.05, **p<0.01, ***p<0.001, ANOVA.